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采用２，４二硝基氟苯作为衍生化试剂，１６种常见人体生理性氨基酸为分析对象，区带毛细管电泳为分离模式，建立一种新的氨基酸衍生化方法。系统研究了反应时间、反应温度和ｐＨ值对衍生化反应的影响。同时观察了４℃和２５℃室温下衍生化产物的稳定性情况，结果发现，无论是在４℃冰箱，还是在２５℃室温下，氨基酸衍生化产物保存５天，紫外吸收值无明显改变。方法的性能指标测定表明，该法的线性在１０～７００μｍｏ１／Ｌ范围内良好（γ在０．９６４～０．９９５之间）。最低检测限（Ｓ／Ｎ＝２）为２．５～７．９μｍｏｌ／Ｌ。测得平均回收率在８６．３～１０７．４％之间。该法具有衍生化反应操作简便、灵敏度高、稳定性好、线性及回收率佳等特点，不仅可用于氨基酸的衍生化反应，而且同样适合于其它含有氨基的生物活性物质的衍生化反应，因而具有广泛的应用前景
Using 2,4 dinitrofluorobenzene as a derivatization reagent, 16 kinds of common human physiological amino acids were analyzed, and the zone capillary electrophoresis was used as the separation mode to establish a new amino acid derivatization method. The effects of reaction time, reaction temperature and pH on the derivatization reaction were studied systematically. At the same time, the stability of the derivatized products at room temperature of 4 ℃ and 25 ℃ was observed. The results showed that there was no significant change in the UV absorbance when the products were stored for 5 days in refrigerator at 4 ℃ or room temperature at 25 ℃. The determination of the performance of the method shows that the linearity of the method is good in the range of 10-700 μmol / L (γ is between 0.964-0.995). The lowest detection limit (S / N = 2) was 2.5 ~ 7.9μmol / L. The average recovery was measured between 86.3 ~ 107.4%. The method has the advantages of simple and convenient derivatization reaction, high sensitivity, good stability, good linearity and good recovery, and can be used not only for the derivatization of amino acids but also for the derivatization reaction of other amino-containing bioactive substances, thus Has a wide range of applications