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采用加乙醇和河沙在试管中振荡30秒钟的方法汰除了自美国引进的1474个向日葵种质品种所带锈菌孢子,而对种子萌芽率及幼苗生活力无影响,成熟种子也不带锈菌.此法可用于检疫汰除和种子检验.
The addition of ethanol and sand to shake in vitro for 30 seconds eliminated 1474 sunflower germplasms from the United States, but did not affect seed germination and seedling vigor, nor did mature seeds This method can be used for quarantine and seed testing.