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Objective:To identify the hepatoprotective and in vitro antioxidant activity of Lumnitzera racemosa(L.racemosa)leaf extract.Methods:Animals in Group 1 served as vehicle control,Group 2 served as liepatoloxin(CCL_4 treated)group,Group 3 served as positive control(Silyinarin)group,and Group 4,5 and 6 served as(75,150 and 300 mg/kg bw p.o.)L.raremosa leaf extract treated groups.Moreover,in vitro antioxidant DPPH,hydroxyl radical scavenging activity(HRSA),NO,ferric reducing antioxidant power(FRAP),lipid hydroperoxide(LPO)and super oxide dismutase(SOD)were also analyzed for the leaf extract.Results:The levels of the serum parameters such as serum glutamic oxaloacetic transaminase(SCOT),serum glutamic pyruvic transaminase(SGPT),alkaline phosphatase(ALP),bilirubin,cholesterol(CHL),sugar and lactate dehydrogenase(LDU)were significantly increased in CCL_4 treated rats when compared with the control group(P<0.05).But the L.racemosa leaf extract treated rats showed maximum reduction of SGOT[(210.36±19.63)IU/L],SGPT[(82.37±13.87)IU/L],ALP[(197.63±23.43)IU/L],bilurubin[(2.15±0.84)mg/dL],cholesterol[(163.83±15.63)mg/dL],sugar[(93.00±7.65)mg/dL]and LDH[(1134.00±285.00)IU/L]were observed with the high dose(300 mg/kg bw)of leaf extract treated rats.Histopathological scores showed that,no visible changes were observed with high dose(300 mg/kg bw)of leaf extract treated rats except few mild necrosis.The IC_(50)values were observed as(56.37±4.87)μg/mL,(57.68±1.98)μg/mL,(64.I5±2.90)μg/mL,(61.94±3.98)μg/mL,(94.53±1.68)μg/mL and(69.7±2.65)μg/mL for DPPH,HRSA,NO,FRAP,LPO and SOD radical scavenging activities,respectively.Conclusions:In conclusion,the hepatoprotective effect of the L.racemosa leaf extract might be due to the presence of phenolic groups,terpenoids and alkaloids and in vitro antioxidant properties.
Group 2 served as liepatoloxin (CCL_4 treated) group, Group 3 served as positive control (Group 1 served as vehicle control), Group 2 served as liepatoloxin (CCL_4 treated) group, Group 3 served as positive control (Silyinarin) group, and Group 4,5 and 6 served as (75,150 and 300 mg / kg bw po) L. raremosa leaf extract treated groups. More over, in vitro antioxidant DPPH, hydroxyl radical scavenging activity Results: The levels of the serum parameters such as serum glutamic oxaloacetic transaminase (SCOT), serum glutamic pyruvic transaminase (SOD) were also analyzed for leaf extract SGPT, alkaline phosphatase (ALP), bilirubin, cholesterol (CHL), sugar and lactate dehydrogenase (LDU) were significantly increased in CCL_4 treated rats when compared with the control group (P <0.05) rats showed maximum reduction of SGOT [(210. 36 ± 19.63 IU / L], SGPT (82.37 ± 13.87) IU / L, ALP 197.63 ± 23.43 IU / L, bilurubin [(2.15 ± 0.84) mg / dL] and cholesterol [(163.83 ± 15.63) mg / dL], sugar [(93.00 ± 7.65) mg / dL] and LDH [(1134.00 ± 285.00) IU / L] were observed with the high dose (300 mg / kg bw) of leaf extract treated rats. scores showed that, no visible changes were observed with high dose (300 mg / kg bw) of leaf extract treated rats except few mild necrosis. IC 50 values were observed as (56.37 ± 4.87) μg / mL, (57.68 ± 1.98 μg / mL, (64.I5 ± 2.90) μg / mL, (61.94 ± 3.98) μg / mL, (94.53 ± 1.68) μg / mL and (69.7 ± 2.65) μg / mL for DPPH, HRSA, NO, FRAP, LPO and SOD radical scavenging activities, respectively. Conclusions: In conclusion, the hepatoprotective effect of the L.racemosa leaf extract might be due to the presence of phenolic groups, terpenoids and alkaloids and in vitro antioxidant properties.