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目的:观察胶质瘤细胞C6中同源盒基因(Hox基因组)的表达,及苯乙酸(PA)对Hox基因组表达的影响。方法:体外培养C6细胞并用PA诱导分化,提取细胞RNA。用Hox基因三对简并引物P1、P2和P3进行逆转录PCR(RT-PCR)。通过计算机图像分析,Hox基因组mRNA的表达水平用Hox基因(组)/β-肌动蛋白(β-actin)灰度比值表示。应用PA前后基因表达的差异用t检验分析。结果:在胶质瘤细胞C6中,P1、P2和P3组Hox基因表达分别为0.8817±0.0731(n=16),0.6825±0.0987(n=9),0.8608±0.0881(n=16);应用PA后,分别为:0.8765±0.0667(n=8),0.8386±0.0811(n=14),0.8937±0.0598(n=11)。应用PA后,P2组Hox基因表达显著增强,与用药前比较,差异显著(P<0.001)。结论:在胶质瘤C6细胞中,PA对P2组Hox基因mRNA水平的表达有明显的上调作用。PA的作用机理与调节胶质瘤细胞转录过程可能有关。
OBJECTIVE: To observe the expression of homologous box gene (Hox genome) in C6 glioma cells and the effect of phenylacetic acid (PA) on Hox genome expression. Methods: C6 cells were cultured in vitro and differentiated with PA to extract cell RNA. Reverse transcription PCR (RT-PCR) was performed with three pairs of degenerate primers Pl, P2 and P3 of the Hox gene. By computer image analysis, the expression level of Hox genomic mRNA was expressed as the gray level ratio of Hox gene / β-actin. Differences in gene expression before and after application of PA were analyzed by t-test. Results: In C6 glioma cells, the expression of Hox gene in P1, P2 and P3 groups were 0.8817 ± 0.0731 (n = 16), 0.6825 ± 0.0987 (n = 9) and 0.8608 ± 0.0881 0.8765 ± 0.0667 (n = 8), 0.8386 ± 0.0811 (n = 14) and 0.8937 ± 0.0598 (n = 11), respectively. After application of PA, the expression of Hox gene in P2 group was significantly increased, which was significantly different from that before treatment (P <0.001). Conclusion: In C6 glioma cells, PA has a significant up-regulation of Hox mRNA expression in P2 group. The mechanism of action of PA may be related to regulating the transcription of glioma cells.