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目的 :检测食管鳞状细胞癌p5 3基因突变与临床病理及预后的相关性。方法 :应用聚合酶链反应—单链构象多态性分析 (PCR—SSCP)法。结果 :42例食管鳞癌中有 16例出现p5 3基因突变 ,突变率为 3 8.1% ;高分化型癌突变率为18.2 % ( 2 /11) ,中分化型癌 2 6.3 % ( 5 /19) ,低分化型癌 75 .0 % ( 9/12 ) ,低分化型癌突变率明显高于中、高分化型癌 (P <0 .0 5 ) ;突变率淋巴结转移者为 5 6.0 % ( 14 /2 5 ) ,无淋巴结转移者为 11.8% ( 2 /17) ,二者差异有显著意义 (P <0 .0 5 ) ;死亡率突变者为 81.3 % ,无突变者为 2 6.9% ,两者差异有显著意义 (P <0 .0 5 )。结论 :突变的p5 3基因可能参与了食管癌的发生与进展 ,并与肿瘤的分化程度、淋巴结转移和预后相关
Objective : To detect the relationship between p53 gene mutation and clinical pathology and prognosis in esophageal squamous cell carcinoma. Methods: The polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) method was used. RESULTS: Of the 42 esophageal squamous cell carcinomas, 16 had p53 mutations with a mutation rate of 38.1%; highly-differentiated carcinomas had a mutation rate of 18.2% (2/11); moderately differentiated carcinomas had 26.3% (5/19). 75.0% (9/12) of poorly-differentiated cancers. The rate of poorly-differentiated cancers was significantly higher than that of moderately-differentiated cancers (P <0.05). The rate of mutations in lymph node metastases was 56.0 % ( 14/25), with no lymph node metastasis, 11.8% (2/17), the difference between the two was significant (P < 0.05), mortality was 81.3%, and no mutation was 26.9%. There was a significant difference between the two (P < 0.05). Conclusion : Mutated p53 gene may be involved in the occurrence and progression of esophageal cancer and related to the degree of tumor differentiation, lymph node metastasis and prognosis