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目的研究p43蛋白抑制新生血管生成是否通过诱导细胞凋亡来实现。方法用不同浓度的p43蛋白(0,10,50,100μg/ml)作用于HUVEC细胞12 h,检测细胞体外管腔形成能力的变化,同时检测细胞周期变化,并通过胞内总半肽天冬酶(caspase)活性检测、caspase3和caspase7基因水平的变化及DNA片段化检测等方法,研究p43蛋白对HUVEC细胞凋亡的影响。结果与结论 p43蛋白可明显抑制HUVEC细胞体外管腔形成,并随着p43蛋白浓度的升高抑制作用不断增强;不同浓度的p43蛋白对HUVEC细胞周期没有明显影响,不能诱导HUVEC细胞凋亡。p43蛋白抑制新生血管的生成不是通过诱导细胞凋亡来实现的。
Objective To investigate whether p43 protein inhibits neovascularization by inducing apoptosis. Methods HUVEC cells were treated with different concentrations of p43 protein (0, 10, 50 and 100 μg / ml) for 12 h, and the changes of cell formation in vitro were observed. Cell cycle changes were also examined. (caspase) activity assay, caspase3 and caspase7 gene level changes and DNA fragmentation detection of p43 protein on HUVEC apoptosis. RESULTS AND CONCLUSION p43 protein could significantly inhibit the formation of lumen in HUVECs in vitro and inhibit the growth of HUVECs with increasing concentration of p43 protein. Different concentrations of p43 protein had no obvious effect on the cell cycle of HUVECs and could not induce the apoptosis of HUVECs. Inhibition of neovascularization by p43 protein is not achieved by inducing apoptosis.