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本文采用3-氨基邻苯二甲酰肼增强化学发光(CL)的方法检测8例特发性肺纤维化(IPE)患者和10例正常对照的肺灌洗液(BAL)全细胞的CL。IPF患者BAL细胞的自发发光显著高于对照(P<0.01),而其刺激发光竟高达对照的11倍(P<0.001),说明IPF患者肺组织的炎症细胞释放大量的反应性氧中间物(ROI),后者可能是造成肺损害的原因。此外还对5例IPF患者接受激素治疗前后BAL细胞的CL进行了比较,3例接受激素后临床明显好转,其BAL细胞的CL较治疗前显著下降(P<0.05),2例治疗后无好转的患者其BAL的CL与治疗前相比无显著性变化(P>0.05)。本文以化学发光的方法研究和探讨了特发性肺纤维化的发病机制。
In this paper, the CL of whole lung of lung lavage fluid (BAL) in 8 patients with idiopathic pulmonary fibrosis (IPE) and 10 normal controls was detected by 3-aminophthalocyanine enhanced chemiluminescence (CL) method. The spontaneous luminescence of BAL cells in IPF patients was significantly higher than that of the control (P <0.01), and the stimulated luminescence was 11 times higher than that of the control (P <0.001), indicating that the inflammatory cells in IPF patients release a large amount of reactive oxygen intermediates ROI), the latter may be the cause of lung damage. In addition, 5 cases of IPF patients before and after hormone treatment of BAL cells were compared CL, 3 cases of clinics after hormone was significantly improved, the CL of BAL cells was significantly lower than before treatment (P <0.05), 2 cases did not improve after treatment Of patients had no significant change in CL of BAL compared with those before treatment (P> 0.05). In this paper, chemiluminescence method to study and explore the pathogenesis of idiopathic pulmonary fibrosis.