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目的:研究双参通冠方对急性心肌梗死大鼠缺血心肌内皮细胞VEGF及上游调控因子HIF-1α表达的影响,探讨双参通冠方防治心肌梗死的作用机制。方法:SD大鼠分为模型组、空白组、双参通冠方低剂量组(2.5 g/kg)、中剂量组(5 g/kg)、高剂量组(7.5 g/kg),采用结扎左冠状动脉前降支建立大鼠心肌梗死模型,灌胃给药2周后,取大鼠的心肌组织,制备心肌组织匀浆,Elisa法检测VEGF含量,Western blot法检测VEGF及上游调控因子HIF-1α的表达。结果:模型组大鼠心肌组织VEGF含量和VEGF、HIF-1α表达高于空白组,差异有统计学意义(P<0.05);双参通冠方低剂量组、中剂量组和高剂量组VEGF含量和VEGF、HIF-1α表达均高于模型组,差异均有统计学意义(P<0.05);双参通冠方低剂量组VEGF含量和VEGF、HIF-1α表达低于双参通冠方中剂量组,双参通冠方高剂量组VEGF含量和VEGF、HIF-1α表达高于双参通冠方中剂量组,差异均有统计学意义(P<0.05)。结论:双参通冠方可增加急性心肌梗死模型大鼠缺血区心肌组织中VEGF含量和上游调控因子HIF-1α表达,促进血管内皮细胞增殖,诱导缺血部位血管新生,对心肌具有保护作用。
Objective: To investigate the effect of Shuangshentongguan Recipe on the expression of VEGF and its upstream regulatory factor HIF-1α in ischemic myocardium of acute myocardial infarction (AMI) rats and to explore the mechanism of Shuangshen Tongguan Recipe in preventing and treating myocardial infarction. Methods: The SD rats were divided into model group, blank group, Shuangshen Tongguan low dose group (2.5 g / kg), middle dose group (5 g / kg) and high dose group (7.5 g / The left anterior descending coronary artery was used to establish the model of myocardial infarction in rats. After 2 weeks of intragastric administration, the myocardial tissue of rats was prepared and the myocardial tissue homogenate was prepared. The content of VEGF was measured by Elisa method. The VEGF and upstream regulatory factor HIF -1α expression. Results: The content of VEGF and the expression of VEGF and HIF-1α in model group were significantly higher than those in blank group (P <0.05). The expression of VEGF in Shuangshen Tong Guan Fang low dose group, middle dose group and high dose group (P <0.05). The expression of VEGF, VEGF and HIF-1α in Shuangshentonggan low-dose group was lower than that in Shuangshentongguan The expression of VEGF, VEGF and HIF-1α in Shuangshentong Guan Fang high-dose group was higher than that in Shuangshentong Guangan medium dose group (P <0.05). Conclusion: Shuangshentongguan decoction can increase the content of VEGF and up-regulate the expression of HIF-1α in ischemic myocardium in acute myocardial infarction rats, promote the proliferation of vascular endothelial cells and induce the angiogenesis in the ischemic area, which has a protective effect on myocardium .