论文部分内容阅读
固相放免分析如AUSAB试剂(Abbott实验室)已普遍地用于检测抗-HBs,同时也广泛用于临床作为证实已患过乙型肝炎的检测方法。然而,新研究领域的发展例如:抗-HBs杂交瘤细胞分泌克隆的筛选以及对乙肝疫苗免疫小动物后所得抗血清的分析需要建立一种可靠的免疫分析方法。以便在用大量材料作分析时减少AUSAB的高昂费川。另外,在测定杂交瘤克隆细胞融合后的早期分泌物时需要比AUSAB试剂更敏感的试剂。
Solid-phase radioimmunoassay, such as the AUSAB reagent (Abbott Laboratories), is commonly used to detect anti-HBs and is also widely used clinically as a test to confirm the presence of hepatitis B. However, the development of new research fields such as the screening of anti-HBs hybridoma cell secreting clones and the analysis of antisera raised after the hepatitis B vaccine immunization of animals require the establishment of a reliable immunoassay. In order to reduce the cost of AUSAB when analyzing large amounts of material. In addition, reagents that are more sensitive than AUSAB reagents are needed to measure early secretions after hybridoma clonal fusion.