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本文以乙醇脱氢酶(ADH)和胆红素氧化酶(BOD)为生物催化剂,以碳纳米管为电极材料,构筑了全酶型乙醇/氧气生物燃料电池.将乙醇脱氢酶负载于单壁碳纳米管(SWCNTs)上,采用亚甲基绿(MG)为NADH的电化学催化剂,实现乙醇的生物电化学催化氧化,制备了生物燃料电池ADH/MG/SWCNTs/GC的电极(阳极).同时,将胆红素氧化酶固定于单壁碳纳米管上,通过其直接电子转移,实现了氧气的生物电化学催化还原,制得生物燃料电池的BOD/SWCNTs/GC阴极.据此构筑了全酶型的无膜生物燃料电池,在空气饱和40 mmol·L-1乙醇磷酸缓冲溶液中该电池开路电压为0.53 V,最大输出功率密度为11μW·cm-2.以商品化伏特酒作为燃料,该生物燃料电池最大输出功率为3.7μW·cm-2.
In this paper, ethanol dehydrogenase (ADH) and bilirubin oxidase (BOD) were used as biocatalysts and carbon nanotubes as electrode materials to construct a holoenzyme-type ethanol / oxygen biofuel cell. The electrode (anode) of biofuel cell ADH / MG / SWCNTs / GC was prepared by electrochemical oxidation of ethanol on the surface of carbon nanotubes (SWCNTs) using NADH as the electrochemical catalyst with methylene green (MG) At the same time, bilirubin oxidase was immobilized on single-walled carbon nanotubes, and by direct electron transfer, bioelectrochemical catalytic reduction of oxygen was achieved to produce BOD / SWCNTs / GC cathodes for biofuel cells. The biofuel cell without membrane biofuel cell has an open circuit voltage of 0.53 V and a maximum output power density of 11 μW · cm-2 in a 40 mmol·L-1 ethanol-saturated air-saturated air buffer solution.The commercial vodka Fuel, the biofuel cell maximum output power of 3.7μW · cm-2.