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【目的】建立人增生性瘢痕 (HTS)裸鼠模型和观察瘢痕组织形态学变化特点。【方法】将HTS移植至裸鼠的皮下 ,采用光镜、电镜和免疫组化方法观察移植后的HTS的形态学、T淋巴细胞、巨噬细胞和转化生长因子 β1(TGF β1)的分布变化特点。【结果】术后裸鼠全部成活 ,植入的瘢痕无坏死。在 6～ 7周内均能保持其原有的组织结构。光镜、电镜观察成纤维细胞 (FB)内含丰富的粗面内质网、线粒体 ;移植后瘢痕组织中的T淋巴细胞、巨噬细胞细胞分布和TGF β1免疫反应强度与移植前相似。【结论】在裸鼠体内建立的HTS模型是一个可行和可靠的动物模型 ,移植的HTS在 6～ 7周内仍能保持其原有的组织形态特征。
【Objective】 To establish a human hypertrophic scars (HTS) nude mouse model and observe the morphological changes of scar tissue. 【Methods】 HTS was transplanted subcutaneously into nude mice. The morphological changes, distribution of T lymphocytes, macrophages and transforming growth factor β1 (TGFβ1) in HTS were observed by light microscopy, electron microscopy and immunohistochemistry Features. 【Results】 All nude mice survived without any necrosis. In 6 to 7 weeks to maintain its original organizational structure. Light microscope and electron microscope showed that fibroblasts (FBs) were rich in rough endoplasmic reticulum and mitochondria. The distribution of T lymphocytes, macrophage cells and TGF-β1 immunoreactivity in scar tissue after transplantation were similar to those before transplantation. 【Conclusion】 HTS model established in nude mice is a feasible and reliable animal model. The transplanted HTS can maintain its original morphological features within 6 ~ 7 weeks.