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实验用Wistar品系大鼠共68只,体重80-110克。中毒组用乙基硫氨酸(D-ethionine)1克/100毫升水溶液按0.5毫升/100克体重胃饲动物,11天、17天、33天。对照组胃饲水。治疗组于动物中毒后用西宁西大黄水浸煎剂0.5克/毫升/100克体重,每日一次治疗8天或14天,另一组不治疗使其自然恢复。动物按计划颈椎脱臼处死后,取肝,胰组织固定于2.5％戊二醛,1％四氧锇中,Epon812包埋、铀-铅染色,透射电镜观察。
A total of 68 experimental Wistar rats, weighing 80-110 g. Toxic animals were intragastrically fed with D-ethionine (1 g / 100 ml) at 0.5 ml / 100 g body weight for 11 days, 17 days and 33 days. Control group stomach water. The treatment group was poisoned with xanthine west rhubarb water decoction 0.5 g / ml / 100 g body weight, once a day for 8 days or 14 days, the other group without treatment to make it natural recovery. Animals were sacrificed after cervical dislocation according to plan. The liver and pancreatic tissues were fixed in 2.5% glutaraldehyde, 1% osmium tetroxide, embedded in Epon812, stained with uranium-lead and observed by transmission electron microscopy.