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目的:使用microRNAs基因芯片及实时定量PCR法测定骨肉瘤组织中miR-15a-5p和miR-16-5p的相对表达含量,并与瘤旁组织对比,分析骨肉瘤细胞内miR-15a-5p和miR-16-5p的表达变化。方法:选取34例骨肉瘤组织蜡块样本,使用microRNAs基因芯片观察miR-15a-5p和miR-16-5p在骨肉瘤和瘤旁组织内的表达差异;实时定量PCR法测定骨肉瘤组织和瘤旁组织中miR-15a-5p和miR-16-5p的相对表达含量,并将两种结果对比分析。结果:microRNAs基因芯片结果显示,在骨肉瘤组织中,miR-15a-5p在肿瘤中的表达较瘤旁组织低1.79倍,miR-16-5p较瘤旁组织低1.62倍。实时定量PCR实验结果表明,miR-15a-5p和miR-16-5p表达较瘤旁组织降低,差异有统计学意义(P<0.05)。经过统计学计算,miR-15a-5p在肿瘤中的表达较瘤旁组织低3.14倍,miR-16-5p较瘤旁组织低5.65倍。结论:在骨肉瘤中,miR-15a-5p和miR-16-5p表达含量降低,提示这两种microRNAs在骨肉瘤中可能做为抑癌因子存在。
OBJECTIVE: To determine the relative expression of miR-15a-5p and miR-16-5p in osteosarcoma tissues by microRNAs microarray and real-time quantitative polymerase chain reaction (PCR) and to compare the expression of miR-15a-5p and miR- Changes in miR-16-5p expression. Methods: The expression of miR-15a-5p and miR-16-5p in osteosarcoma and para-tumor tissues was detected by microRNAs microarray in 34 cases of osteosarcoma tissue samples. Real-time quantitative PCR was used to detect the expression of miR-15a-5p and miR- The relative expression levels of miR-15a-5p and miR-16-5p in adjacent tissues were compared and analyzed. Results: The results of microRNAs microarray showed that in osteosarcoma, the expression of miR-15a-5p in tumor was 1.79 times lower than that in tumor-adjacent tissue and miR-16-5p was 1.62 times lower than that in tumor-adjacent tissue. Real-time quantitative PCR results showed that the expression of miR-15a-5p and miR-16-5p was lower than that of tumor-adjacent tissues (P <0.05). After statistical analysis, the expression of miR-15a-5p in tumor was 3.14 times lower than that in tumor-adjacent tissues, and miR-16-5p was 5.65-fold lower than that in tumor-adjacent tissues. CONCLUSIONS: The decreased expression of miR-15a-5p and miR-16-5p in osteosarcoma suggests that these two microRNAs may be potential tumor suppressors in osteosarcoma.