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目的:对野生三角叶黄连进行遗传多样性研究。方法:通过ISSR技术对8个野生三角叶黄连居群共90个个体进行遗传多样性分析。结果:用12个随机引物共扩增出128条清晰条带,其中94条具多态性,平均多态性位点比率为73.44%,Ne i’s基因多样性指数H=0.192 5,Shannon’s多样性指数I=0.302 8,遗传分化指数Gst=0.721 2,遗传距离和遗传一致度分别为0.085 8~0.231 4,0.804 6~0.942 5。结论:三角叶黄连种水平上具有较高的遗传多样性,遗传变异主要存在于居群间,遗传多样性与地理关系表现出明显的相关性,ISSR可以作为研究遗传多样性及遗传分化的有效标记。
Objective: To study the genetic diversity of Coptis chinensis. Methods: The genetic diversity of 90 individuals of Coptis chinensis populations from 8 wild populations was analyzed by ISSR. Results: Twelve random primers were used to amplify 128 clear bands, of which 94 were polymorphic, the average polymorphic loci ratio was 73.44%, Ne i’s gene diversity index was 0.192 5, Shannon’s diversity Index I = 0.302 8, genetic differentiation index Gst = 0.721 2, genetic distance and genetic identity were 0.085 8 ~ 0.231 4,0.804 6 ~ 0.942 5 respectively. Conclusion: The genetic diversity of Coptis chinensis has high genetic diversity. The genetic variation mainly exists in the populations. The genetic diversity shows a significant correlation with geographical relationship. ISSR can be used as a marker to study genetic diversity and genetic differentiation mark.