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报道从２３７枚家兔胚胎中建成７个可连续传代的ＥＳ样细胞系。建系条件为，使用小鼠原始胚胎成纤维细胞（ＭＥ）作饲养层，以含１０％胎牛血清和１０％兔血清的ＤＭＥＭ／Ｆ１２为培养基，添加白血病抑制因子（ＬＩＦ）或上皮生长因子（ＥＧＦ），胚龄为９０，９６ｈ。该细胞系的细胞，在许多方面类似于小鼠ＥＳ细胞：具干细胞的形态特征，呈集落型生长，可连续传代并保持其形态特征，具一定的自发分化和诱导分化的能力，悬浮培养可形成简单类胚，但皮下接种同种乳兔未获肿瘤。用所建的兔ＥＳ样细胞进行卵母细胞核移植时，有１１％发育为囊胚
Seven ES-like cell lines that can be passaged serially were reported from 237 rabbit embryos. The establishment of the conditions for the use of mouse embryonic fibroblasts (ME) as a feeder layer, with 10% fetal bovine serum and DMEM / F12 as a medium, add leukemia inhibitory factor (LIF) or epithelial growth Factors (EGF), embryonic age 90,96 h. Cells of this cell line resemble mouse ES cells in many ways: the morphological features of stem cells are colony-type growth, which can be continuously passaged and maintain its morphological characteristics, with certain spontaneous differentiation and differentiation-inducing ability. Suspension culture can be The formation of a simple embryoid, but the same kind of rabbit subcutaneously vaccinated without tumor. 11% developed as blastocysts when oocytes were nuclear transplanted with rabbit ES-like cells