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目的研究血清类黏蛋白1样蛋白3(ORMDL3)和E26转录因子1(Ets-1)基因在反复喘息患儿外周血中的表达以及两者的相关性。方法采用RT-PCR法检测非过敏性正常对照婴幼儿(A组,24例)和反复喘息患儿(B组,25例)外周血中ORMDL3和Ets-1mRNA表达水平。结果B组外周血ORMDL3和Ets-1mRNA表达水平高于A组(P<0.05);ORMDL3与Ets-1基因表达水平呈正相关(r=0.7704,P<0.05)。结论 ORMDL3和Ets-1基因的表达水平与患儿的反复喘息密切相关。
Objective To investigate the expression of the genes of the serotypes of mucin-like protein 3 (ORMDL3) and E26 transcription factor 1 (Ets-1) in the peripheral blood of patients with recurrent wheezing and their correlation. Methods RT-PCR was used to detect the mRNA expression of ORMDL3 and Ets-1 in peripheral blood of non-allergic normal infants (group A, 24 cases) and recurrent wheezing children (group B, 25 cases). Results The expression of ORMDL3 and Ets-1 mRNA in peripheral blood of group B was higher than that of group A (P <0.05). The expression of ORMDL3 and Ets-1 was positively correlated (r = 0.7704, P <0.05). Conclusion The expression of ORMDL3 and Ets-1 gene is closely related to repeated wheezing in children.