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生物体内大多数生物过程在某种意义上与色谱的作用机理类似, 因此我们提出一个色谱新概念,即利用仿生固定相和流动相模拟生物过程。目前色谱的应用主要局限在它的分离和定量功能。如果使固定相具有生物特性,即利用色谱模拟生物过程,将不仅进一步拓展色谱的应用空间,而且也可以解决大量的生命科学中的问题。生物功能化色谱尚未见报道,这个新技术不仅可以迅速、准确地测定生物特性,同时结合色谱的分离能力可以实现高通量测定。在本研究中我们发展了用于模拟多巴胺代谢的生物功能化色谱。根据影响多巴胺在体内代谢过程的相关酶以及它们的作用机理,通过构筑具有生物活性的色谱固定相和流动相把生物分子之间的作用过程用色谱模拟出来。这个具有生物功能的色谱已经尝试性地用于帕金森病的药物筛选。
Most biological processes in the organism resemble chromatographic mechanisms in a sense, so we propose a new concept of chromatography that uses biomimetic stationary phases and mobile phases to model biological processes. The current application of chromatography is mainly limited to its separation and quantitative functions. If you make the stationary phase biological, that the use of chromatography to simulate biological processes, will not only further expand the chromatographic application of space, but also can solve a large number of life science problems. Biofunctional chromatography has not been reported yet. This new technique not only allows for the rapid and accurate determination of biological properties, but also enables the high-throughput determination of chromatographic separations. In this study we developed a biofunctional chromatogram for mimicking dopamine metabolism. According to the related enzymes that affect the metabolic process of dopamine in the body and their mechanism of action, the interaction between biomolecules through the construction of bioactive chromatographic stationary phase and mobile phase was simulated by chromatography. This biologically functional chromatography has tentatively been used for drug screening of Parkinson’s disease.