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[目的]研究食管鳞癌肿瘤组织及外周血多基因甲基化状态,以及不同基因甲基化的相关性。[方法]应用real-time MSP技术对76例食管鳞癌患者肿瘤组织、配对的癌旁正常组织、术前外周血中APC、RARβ2、CDH1、p16INK4α、RASSF1A抑癌基因的甲基化状态进行检测。随机选取60名年龄配对的健康志愿者外周血浆DNA作对照。[结果]肿瘤组织APC、RARβ2、CDH1、p16INK4α、RASSF1A的甲基化率显著高于对应癌旁正常组织(P=0.000)。术前外周血中这5种基因的甲基化率显著高于健康对照组(P=0.000)。RARβ2、CDH1、p16INK4α、RASSF1A甲基化有显著性相关,APC与这4个基因甲基化无相关性。[结论]食管癌患者癌组织及外周血抑癌基因APC、RARβ2、CDH1、p16INK4α、RASSF1A高甲基化,RARβ2、CDH1、p16INK4α、RASSF1A甲基化有显著相关性。
[Objective] To investigate the multi-gene methylation status of esophageal squamous cell carcinoma and peripheral blood and the correlation between methylation of different genes. [Methods] The methylation status of APC, RARβ2, CDH1, p16INK4α and RASSF1A tumor suppressor genes in 76 cases of esophageal squamous cell carcinoma tissues, matched paracancerous normal tissues and preoperative peripheral blood were detected by real-time MSP . Randomly selected 60 age-matched healthy volunteers peripheral plasma DNA as a control. [Results] The methylation rates of APC, RARβ2, CDH1, p16INK4α and RASSF1A in tumor tissues were significantly higher than those in corresponding normal tissues (P = 0.000). The methylation rates of these five genes in preoperative peripheral blood were significantly higher than those in healthy controls (P = 0.000). There was a significant correlation between methylation of RARβ2, CDH1, p16INK4α and RASSF1A, and no correlation between APC and methylation of these 4 genes. [Conclusion] The methylation of APC, RARβ2, CDH1, p16INK4α, RASSF1A hypermethylation and RARβ2, CDH1, p16INK4α, RASSF1A methylation in esophageal cancer patients is significantly correlated.