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以中国仓鼠卵巢(CHO)细胞为材料,检测了三-(2-甲基-1氮丙啶)磷化氧(MAPO)的诱变作用,结果表明,在加S9活化与不加S9活化条件下,MAPO在0.1～50.0μg·ml~1浓度范围内,可诱发CHO细胞姐妹染色单体互换率及染色体畸变率升高而在1.0～100.0μg·ml~1浓度范围内,可引起微核率明显升高,并诱发次黄嘌呤鸟嘌呤磷酸核糖基转移酶位点发生正向突变,所有结果均具有较好的剂量反应关系,表明MAPO具有很强的诱变作用。
The Chinese hamster ovary (CHO) cells were used as materials to test the mutagenicity of MAPO. The results showed that under the conditions of S9 activation and without S9 activation , The concentration of MAPO in the range of 0.1 ~ 50.0μg · ml ~ 1 could induce the increase of sibling chromatid exchange rate and chromosome aberration rate in CHO cells, and the concentration of MAPO in the range of 1.0 ~ 100.0μg · ml ~ The nuclear rate was significantly increased, and hypoxanthine guanine phosphoribosyltransferase site induced positive mutation, all the results have a good dose-response relationship, indicating that MAPO has a strong mutagenic effect.