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目的:探讨γ-生育三烯酚(γ-T3)对人胃癌细胞(SGC-7901和MGC-803)迁移和侵袭能力的影响。方法:不同浓度的γ-T3对SGC-7901和MGC-803细胞作用24h后,采用CCK-8法、迁移试验、Transwell小室侵袭试验和酶联免疫吸附试验(ELISA)研究γ-T3对细胞增殖、迁移能力和侵袭能力,以及基质金属酶(MMPs)表达量的影响。结果:CCK-8的结果显示,γ-T3对SGC-7901和MGC-803细胞有抑制作用,并具有剂量-效应关系;体外划痕试验的结果显示,随着药物浓度增加,迁移至划痕区的细胞逐渐减少,60μmol/L组仅有少许细胞迁移至划痕区。在MGC-803细胞中F=25.55,P=0.00;在SGC-7901细胞F=36.15,P=0.00,差异有统计学意义;Transwells小室侵袭实验结果显示,肿瘤细胞侵袭穿透Matrigel胶和Transwell小室微孔膜的能力明显下降,穿膜细胞数目随着药物浓度增加明显减少,在MGC-803细胞中F=23.53,P=0.00;在SGC-7901细胞F=26.00,P=0.00,差异均有统计学意义;ELISA的结果显示,细胞培养上清液中MMP-2和MMP-9分泌量随药物浓度增加而降低。在MGC-803细胞MMP-2中F=62.41,P=0.00;MMP-9中F=314.18,P=0.00。在SGC-7901细胞MMP-2中F=52.58,P=0.00;MMP-9中F=70.00,P=0.00,差异均有统计学意义。结论:γ-T3可显著抑制SGC-7901和MGC-803细胞的增殖、迁移和侵袭,其机制可能与选择性抑制SGC-7901和MGC-803细胞分泌MMP-2和MMP-9有关。
Objective: To investigate the effect of γ-tocotrienol (γ-T3) on the migration and invasion of human gastric cancer cells (SGC-7901 and MGC-803). Methods: The effects of γ-T3 on proliferation of SGC-7901 and MGC-803 cells were studied by CCK-8 assay, transwell assay, Transwell chamber invasion assay and enzyme linked immunosorbent assay (ELISA) , Migration and invasion ability, and matrix metalloproteinase (MMPs) expression. Results: The results of CCK-8 showed that γ-T3 had inhibitory effect on SGC-7901 and MGC-803 cells with dose-effect relationship. The results of in vitro scratch test showed that the migration to scratch increased with the increase of drug concentration In the 60μmol / L group, only a few cells migrated to the scratch area. In MGC-803 cells, F = 25.55, P = 0.00; in SGC-7901 cells F = 36.15, P = 0.00, the difference was statistically significant; Transwells cell invasion assay showed that the invasion of tumor cells penetrated Matrigel and Transwell chamber The number of transmembrane cells decreased significantly with the increase of drug concentration, F = 23.53, P = 0.00 in MGC-803 cells; F = 26.00, P = 0.00 in SGC-7901 cells, the difference was The results of ELISA showed that the secretion of MMP-2 and MMP-9 in the cell culture supernatant decreased with the increase of the drug concentration. F = 62.41, P = 0.00 in MMP-2 of MGC-803 cells; F = 314.18, P = 0.00 in MMP-9. F = 52.58, P = 0.00 in MMP-2 of SGC-7901 cells; F = 70.00, P = 0.00 in MMP-9, the differences were statistically significant. Conclusion: γ-T3 can significantly inhibit the proliferation, migration and invasion of SGC-7901 and MGC-803 cells, which may be related to the selective inhibition of the secretion of MMP-2 and MMP-9 by SGC-7901 and MGC-803 cells.