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观察N-乙酰半胱氨酸(NAC)对矽肺大鼠早期炎症介质肿瘤坏死因子-α(TNF-α)、白细胞介素-8(IL-8)和高敏C-反应蛋白(hsCRP)的干预作用,并探讨炎症因素在矽肺发病过程中的作用。选取Wistar大鼠为研究对象,随机分为模型组、治疗组、对照组3组,每组32只。模型组以气管滴注二氧化硅混悬液建立矽肺大鼠模型。治疗组以大剂量NAC在成模前一周进行每天灌胃干预治疗。分别在染尘后的第3、7、14、28天分批处死大鼠取材。切取肺组织标本作病理切片,行苏木精-伊红(HE)染色,Masson染色,TNF-α免疫组织化学染色,观察肺组织病理变化,用酶联免疫法(ELISA)测定血清和支气管肺泡灌洗液(BALF)中TNF-α和IL-8的含量,用散射比浊法测定hsCRP含量。模型组大鼠血清和BALF中TNF-α、IL-8、hsCRP的浓度明显高于对照组,差异有统计学意义(P<0.05或P<0.01)。与模型组相比较,治疗组各时间点血清和BALF中TNF-α、IL-8和hsCRP浓度均低于模型组,差异具有统计学意义(P<0.05或P<0.01)。结果表明,在矽肺大鼠模型中,大剂量NAC有助于减轻血清和BALF中IL-8、TNF-α、hsCRP等炎症介质的升高,提示NAC可能有助于延续肺纤维化的发生。
To observe the effects of NAC on early inflammatory mediators TNF-α, IL-8 and hsCRP in silicosis rats Role and explore the role of inflammatory factors in the pathogenesis of silicosis. Wistar rats were selected as study subjects and randomly divided into model group, treatment group and control group with 32 rats in each group. The model group was established by tracheal instillation of silica suspension. The treatment group with high-dose NAC in the week before the model for intragastric intervention. Rats were sacrificed in batches on the 3rd, 7th, 14th and 28th days after the infection respectively. The lung tissue specimens were cut out for pathological examination. HE staining, Masson staining and TNF-α immunohistochemical staining were performed to observe the pathological changes in the lung tissue. Serum and bronchoalveolar lavage were detected by enzyme-linked immunosorbent assay (ELISA) The contents of TNF-α and IL-8 in lavage fluid (BALF) were determined by nephelometry. The concentrations of TNF-α, IL-8 and hsCRP in the serum and BALF in the model group were significantly higher than those in the control group (P <0.05 or P <0.01). Compared with the model group, the concentrations of TNF-α, IL-8 and hsCRP in the serum and BALF at each time point in the treatment group were lower than those in the untreated group, with statistical significance (P <0.05 or P <0.01). The results showed that high-dose NAC could reduce the increase of inflammatory mediators such as IL-8, TNF-α, hsCRP in serum and BALF in the silicosis rat model, suggesting that NAC may help to prolong the occurrence of pulmonary fibrosis.