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通过ＲＴ－ＰＣＲ从庚型肝炎患者血清中扩增出庚型肝炎病毒Ｅ１区５′端基因Ｅｐ，经限制性内切酶消化后克隆入ｐＵＣ１９质粒中。序列分析结果表明，该基因与中国河北株相关基因核苷酸的同源性为９２％。将该基因亚克隆入原核表达载体ｐＥＸ３１ｂ中，成功地构建了ｐＥＸ３１ｂ－Ｅｐ重组质粒，经４２℃诱导表达，在Ｍｒ２００００处可见一条明显的表达带，表达产物约占总菌体蛋白的８％。经蛋白铜染洗脱法纯化蛋白，ＥＬＩＳＡ检测表明，该蛋白具有抗原性。
The gene Ep of the 5 ’end of Hepatitis G virus E1 region was amplified by RT-PCR from the serum of patients with hepatitis G, cloned into pUC19 plasmid by restriction endonuclease digestion. The results of sequence analysis showed that the homology of this gene with that of Chinese Hebei strain was 92%. The gene was subcloned into the prokaryotic expression vector pEX31b. The pEX31b-Ep recombinant plasmid was successfully constructed and expressed at 42 ℃. A clear expression band was found at Mr20000, which accounted for about 8% of the total bacterial proteins. Proteins were purified by protein copper staining and ELISA showed that the protein was antigenic.