论文部分内容阅读
目的 :探讨慢性乙型肝炎患者血清学标志、HBVDNA载量及HBV前C/C变异与临床病变的关系。方法 :随机选取慢性乙型肝炎患者 2 4 6例 ,其中轻度 10 2例、中度 88例、重度 5 6例 ,分别应用ELISA、实时PCR法检测其血清中的HBV免疫学标志及HBVDNA含量。同时对HBeAg阴性而HBVDNA含量高拷贝的 3例轻度、6例中度及 8例重度患者血清 ,应用巢式PCR方法分离前C/C区全长基因 ,纯化后克隆入T载体 ,鉴定后进行序列测定。结果 :10 2例轻度、88例中度及 5 6例重度患者中血清学标志HBsAg ,HBeAg,抗 -HBc阳性模式分别为 5 3例 (5 1.9% )、4 6例(5 2 .3% )、33例 (5 8.9% ) ;HBsAg ,抗 -HBe,抗 -HBc阳性模式分别为 37例 (36 .3% )、32例 (37.5 % )、2 1例 (37.5 % ) ;单独HBsAg阳性为 8例 (7.8% )、5例 (5 .6 % )、1例 (1.7% ) ;其他模式分别为 4例、5例、1例。HBVDNA含量平均值分别为 10E 5 .5 3拷贝 /ml、10E 6 .0 3拷贝 /ml、10E 6 .5 8拷贝 /ml。HBeAg阴性而HBVDNA含量 >10E 6拷贝 /ml的病例数分别为 3例、6例、8例。统计分析表明 ,轻、中、重慢性乙肝患者间血清学标志及HBVDNA含量差异均不具统计学意义 ,而HBeAg阴性HBVDNA含量高拷贝的患者出现频率差异具有统计学意义。序列测定结果显示 17例患者血清的HBV分离株的前C/C
Objective: To investigate the relationship between serum HBV markers, HBV DNA load, pre-HBV C / C variation and clinical pathology in patients with chronic hepatitis B Methods: Totally 246 cases of patients with chronic hepatitis B were randomly selected, of which 102 were mild, 88 were moderate and 56 were severe. ELISA and real-time PCR were used to detect the serum immunological markers and HBV DNA . At the same time, the full-length pre-C / C gene was isolated by nested PCR from 3 mild, 6 moderate, and 8 severe HBeAg-negative HBeAg-positive sera. After purification, it was cloned into T vector and identified Sequencing was performed. Results: The positive rates of serological markers HBsAg, HBeAg and anti-HBc in 102 mild cases, 88 moderate and 56 severe cases were 53 (5.9%), 46 (52.3 37 cases (36.5%), 32 cases (37.5%) and 21 cases (37.5%) respectively. HBsAg, anti-HBe and anti-HBc positive model were only HBsAg Positive were 8 cases (7.8%), 5 cases (5.6%) and 1 case (1.7%). The others were 4 cases, 5 cases and 1 case. The average HBVDNA content was 10E5.53 copies / ml, 10E6.0 3 copies / ml, 10E6.58 copies / ml, respectively. The number of cases with HBeAg negative and HBVDNA> 10E 6 copies / ml were 3, 6 and 8 respectively. Statistical analysis showed no significant difference in the serological markers and HBVDNA levels among patients with mild, moderate or severe chronic hepatitis B, while the frequency of HBeAg-negative patients with high HBVDNA copies was statistically significant. Sequencing results showed pre-C / C of HBV isolates from sera from 17 patients