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目的了解嘉善县成人病毒性腹泻病原的流行特征。方法采集2014年6月-2015年5月在嘉善县第一人民医院肠道门诊就诊患者的粪便标本;采用实时荧光逆转录PCR法检测标本中轮状病毒(RV)、诺如病毒Ⅰ型(NV GⅠ)、诺如病毒Ⅱ型(NV GⅡ)和星状病毒(Ast V)核酸,并对结果和患者信息进行统计分析。结果共收集408份标本,肠道病毒总阳性率为31.62%,NV GⅡ阳性率最高(28.19%),其次为RV(3.19%),NV GⅠ未检出;检出病毒构成中,NV GⅡ占比最高(87.79%);男性不同年龄段检出率差异有统计学意义(χ2=15.38,P<0.05),40岁~49岁检出率最高(55.56%),<20岁最低(11.76%);病毒检出高峰在4月和12月,6月无阳性检出。结论 NV GⅡ是嘉善县病毒性腹泻主要病原,成人各年龄段都可感染,应加强冬春季病毒性腹泻疾病的监测。
Objective To understand the prevalence of adult viral diarrhea in Jiashan County. Methods The stool specimens of patients in the Intestinal Clinic of First People’s Hospital of Jiashan from June 2014 to May 2015 were collected. Real-time fluorescence reverse transcription PCR was used to detect the expression of rotavirus (RV), Norovirus type Ⅰ NV GⅠ), Norovirus Ⅱ (NV GⅡ) and Astrovirus (Ast V) nucleic acids, and the results and patient information were statistically analyzed. Results A total of 408 specimens were collected. The total positive rate of enterovirus was 31.62%, the highest positive rate of NV GⅡ was 28.19%, the second was RV (3.19%), while the second was NV GⅠ. (Χ2 = 15.38, P <0.05), the highest detection rate was 40.54 years old (55.56%), the lowest was 20 years old (11.76%), the highest was (87.79% ); The peak of virus detection in April and December, June, no positive detection. Conclusion NV G Ⅱ is the main pathogen of viral diarrhea in Jiashan County. It can be infected in all age groups and the surveillance of viral diarrhea in winter and spring should be strengthened.