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对香蕉枯萎病病菌粗毒素进行高温高压(121℃,0.103MPa)、不同pH值、紫外线照射以及甲醇沉淀处理后,测定其致病活性的变化,以明确其基本理化性质。结果表明:该毒素具有很强的热稳定性,经高温高压处理20min后仍然保持强的致病活性;偏酸(pH3~6)或偏碱(pH8~10)条件均会降低毒素的致病活性;紫外线照射对该毒素的毒性具有削弱作用;经甲醇沉淀处理后,上清液具有致病活性,而沉淀不具有致病活性:说明该毒素属于极性较高的一类小分子非蛋白物质。香蕉枯萎病菌粗毒素对不同种类作物种子的萌发和胚芽生长都有不同程度的抑制作用,是一种非寄主专化性毒素。
The crude toxins of banana wilt disease were assayed for their pathogenicity under high temperature and high pressure (121 ℃, 0.103MPa), different pH values, UV irradiation and methanol precipitation to confirm their basic physical and chemical properties. The results showed that the toxin had strong thermostability and maintained strong pathogenicity after being treated with high temperature and high pressure for 20 minutes. The conditions of partial acid (pH3 ~ 6) or partial alkali (pH8 ~ 10) Activity; ultraviolet irradiation has a weakened effect on the toxicity of the toxin; after the methanol precipitation treatment, the supernatant has a pathogenic activity, and the precipitate does not have pathogenic activity: that the toxin belongs to a class of higher polarity of a small molecule of non-protein substance. Fusarium banana Fusarium solani has different degrees of inhibition on germination and germ growth of different kinds of crop seeds and is a non-host specific toxin.