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目的以B淋巴细胞刺激因子受体BAFF-R基因5’上游区序列为研究对象,初步鉴定、分析该基因的启动子所在区域。方法克隆BAFF-R基因5’侧翼区1823 bp序列,构建8个含有不同长度启动子序列的荧光素酶报告基因表达质粒pGL3-B1～B8,将这8个序列缺失重组质粒与psv-β-gal质粒共转染细胞,检测荧光素酶的相对活性,确定启动子所在区域。结果8个重组质粒中pGL3-B2、pGL3-B3、pGL3-B5、pGL3-B6启动子的活性较低;pGL3-B7启动子的活性最强,pGL3-B8启动子活性最低。结论BAFF-R基因5’端-288～-430、-712～-868和-1420～-1562三个区段可能存在转录沉默子元件,-617～-712和-1277～-1420区段存在转录增强子元件,BAFF-R基因的核心启动子可能位于-1420～+261区域。
OBJECTIVE: To study the 5 ’upstream region of BAFF-R gene of B lymphocyte stimulating factor receptor gene, initially identify and analyze the promoter region of this gene. Methods The 1823 bp sequence of 5 ’flanking region of BAFF-R gene was cloned, and 8 luciferase reporter gene expression plasmids pGL3-B1 ~ B8 containing different length promoter sequences were constructed. The 8 recombinant plasmids deleted the recombinant plasmid and psv- gal plasmid co-transfected cells to detect the relative activity of luciferase, to determine the promoter region. Results The activity of pGL3-B2, pGL3-B3, pGL3-B5 and pGL3-B6 in the 8 recombinant plasmids was low. The activity of pGL3-B7 was the strongest and the activity of pGL3-B8 was the lowest. Conclusion There may exist transcriptional silencers in the three regions of -2FF ~ -430, -712 ~ -868 and -1420 ~ -1562 of BAFF-R gene. The -617 ~ -712 and -1277 ~ -1420 segments exist Transcriptional enhancer element, the core promoter of BAFF-R gene may be located in the -1420 ~ +261 region.