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目的:针对自主研制的GA118-16A遗传分析仪,设计一系列的检测方法以实现对仪器性能的评价。方法:从仪器的迁移速度、灵敏度、重复性、分辨力等方面进行检测方法设计,并进行试验检验。结果:以Gene Scan-500 LIZ为标准物质,根据迁移速度与电场强度关系,在电泳电压大于13KV时可在规定时间内完成500bp片段长度电泳,并进行有效分离,RSD≤2%;仪器的最低上样检测灵敏度可达3.8×10-2ng/μL;能准确分辨1bp差异的DNA片段;重复性RSD≤0.8%。结论:通过设计的实验方法可以对该分析仪的迁移速度、灵敏度等各项性能指标进行较好的评价。
OBJECTIVE: To design a series of detection methods for GA118-16A genetic analyzer independently developed to evaluate the performance of the instrument. Methods: The test methods were designed from the aspects of instrument migration speed, sensitivity, repeatability, resolution and so on, and tested. Results: Gene Scan-500 LIZ was used as the reference substance. According to the relationship between migration velocity and electric field intensity, electrophoresis voltage greater than 13KV could be used to complete 500bp fragment length electrophoresis within the specified time, and RSD≤2%; the lowest The detection sensitivity of up to 3.8 × 10-2ng / μL; DNA fragments can accurately distinguish 1bp differences; repeatability RSD ≤ 0.8%. Conclusion: Through the design of experimental methods, the analyzer’s migration speed, sensitivity and other performance indicators can be better evaluated.