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以逆行追踪与免疫细胞化学相结合法，探讨了大鼠提睾肌、盲肠系膜和耳廓等微循环研究常用部位的神经肽Ｙ（ＮＰＹ）、降钙素基因相关肽（ＣＧＲＰ）、甲啡呔（Ｍ－ＥＮＫ）和５－羟色胺（５－ＨＴ）等肽能和胺能神经的支配。结果表明：支配提睾肌的运动和感觉神经元分别含有５－ＨＴ和ＣＧＲＰ。支配提睾肌血管、盲肠系膜及其血管的交感节神经元，一部分含有ＮＰＹ，一部分含有Ｍ－ＥＮＫ；支配耳廓局部的运动和感觉神经元均含有ＣＧＲＰ，而支配该部的交感节后神经元则分别含有ＮＰＹ、ＣＧＫＰ和５－ＨＴ。上述结果提示：提睾肌、盲肠系膜和耳廓局部除受经典递质的神经支配之外，ＮＰＹ、ＣＧＲＰ、Ｍ－ＥＮＫ和５－ＨＴ等也同样参与了上述各部位的运动、感觉以及自主性功能的调节活动。
The combination of retrograde tracing and immunocytochemistry was used to investigate the effects of NPY, CGRP, (M-ENK) and serotonin (5-HT) and other canine and amygdala innervation. The results show that motor and sensory neurons that dominate the cremaster muscle contain 5-HT and CGRP, respectively. The sympathetic ganglion neurons that control the spinocerebellar, cecal mesangial and blood vessels, some of which contain NPY and some of which contain M-ENK; the motor and sensory neurons that innervate the auricles contain CGRP, while the sympathetic postganglionic nerve The elements contain NPY, CGKP and 5-HT, respectively. The above results suggest that NPY, CGRP, M-ENK and 5-HT are also involved in the motility, sensation, and autonomy of the above-mentioned parts in addition to the innervation of the classic neurotransmitter in the cremasteric, cecum mesangial and auricle areas Sexual function adjustment activities.