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应用计算机选择三波长组合须先在 UV-250(或 UV-300)分光光度计上打印出待测组份和干扰组份各自的吸收值,然后在 TRS-80微处理机上用“UV-TwS.1”程序完成挑选最佳三波长组合的工作,输出前10组|△A|最大的三波长组合。根据挑选波长组合在751G 分光光度计上精选确定后再进行测定。所得工作曲线及回收率数据用“UV—TWC、2”程序进行处理。对盐酸氯丙嗪注射液,按前述步骤在手动式(751G)分光光度计上用三波长法进行了测定,平均回收率为100.3±0.28%。五批结果均符合中国药典的要求,标准差在1%以内。文中还对从计算机上选出的126个组合中,选取的不同|△A|值的5组波长组合进行了工作曲线和回收率的测定比较。
Using a computer to select the three-wavelength combination, the UV-250 (or UV-300) spectrophotometer is used to print the absorbance of each of the components to be tested and the interfering components. The absorbance is then measured on a TRS-80 microprocessor using UV- .1 “program to select the best three-wavelength combination of the work, the first 10 output | △ A | maximum three-wavelength combination. Selected according to the selected wavelength combination in the 751G spectrophotometer after the determination of determination. The resulting working curve and recovery data were processed using the ”UV-TWC, 2" program. Chlorpromazine hydrochloride injection, according to the above steps in the manual (751G) spectrophotometer with three-wavelength method was measured, the average recovery was 100.3 ± 0.28%. Five batches of results are in line with the requirements of the Chinese Pharmacopoeia, the standard deviation of less than 1%. The paper also compares the working curve and the recovery rate of five combinations of the selected | ΔA | values of the 126 combinations selected from the computer.