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普通野生稻（ Ｏｒｙｚａｒｕｆｉｐｏｇｏｎ Ｇｒｉｆｆ．）的基因资源对水稻的育种起着至关重要的作用。报道了从其硅胶干燥的小量叶片中制备ＤＮＡ的方法。用此方法制备的ＤＮＡ分子量大（４０～４５ ｋｂ） ，产率也较高（５０ ～２００ μｇ／ｇ） ，且成功地进行了ＲＡＰＤ扩增。用制备的４４ 个居群，１１６８ 个个体的总ＤＮＡ 建立了中国普通野生稻的总ＤＮＡ 库作长期冷冻保存，可用于基于ＰＣＲ 的ＤＮＡ水平上的各种目的的研究。根据实验结果，从在室温下贮存１ 周、３ 个月、６ 个月、１ 年的硅胶干燥的叶片中提取的ＤＮＡ 用于ＲＡＰＤ扩增所得的扩增产物没有差异；模板ＤＮＡ浓度在３ ．１ ～５０ ｎｇ 的范围内均得到很好的ＲＡＰＤ扩增结果。这说明了从硅胶干燥的叶片中提取的普通野生稻的ＤＮＡ 用于ＲＡＰＤ扩增的产物很稳定，将其用于群体遗传分析具有很好的可比性和可靠性。同时也讨论了模板ＤＮＡ的纯度和浓度对ＲＡＰＤ扩增的影响
The genetic resources of Oryzarufipogon Griff. Play a crucial role in the breeding of rice. A method of preparing DNA from a small amount of dried silica gel leaves is reported. The DNA prepared by this method has high molecular weight (40-45 kb) and high yield (50-200 μg / g), and RAPD amplification has been successfully carried out. The total DNA of 44 Chinese populations and 1168 individuals was used to establish a long-term cryopreservation of the common DNA library of O. japonicus, which can be used for various purposes based on the DNA level of PCR. According to the results of the experiment, DNA extracted from the dried silica gel leaves stored at room temperature for 1 week, 3 months, 6 months and 1 year did not differ from the amplification products obtained by RAPD amplification; the template DNA concentration was 3. A good RAPD amplification result was obtained in the range of 1 to 50 ng. This shows that the DNA extracted from common wild rice from the dried leaves of silica gel is very stable for RAPD amplification and has good comparability and reliability for population genetic analysis. The effect of template DNA purity and concentration on RAPD amplification was also discussed