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目的 比较 4种检测严重急性呼吸综合征冠状病毒 (SARS CoV)基因、抗原和抗体的试剂盒在SARS患者早期实验室诊断的可能作用。方法 用 3种酶联免疫吸附试验 (ELISA)分别检测SARS CoVIgG、SARS CoVIgM 和SARS CoVN蛋白 ,用荧光定量聚合酶链反应 (F PCR)检测SARS CoV核酸。结果 根据 16 2份血清的检测结果 ,发病 1- 5天 ,N抗原的阳性率可达 90 .2 % (5 5 /6 1) ;发病后的第 15 - 18天IgG和IgM阳性率为92 .8% (13/ 14 ) ;根据 82份咽拭子的检测结果 ,F PCR法在发病后 1- 5天的阳性率可达 5 6 .3% (14 / 2 4 ) ,6 - 9天可达 71.4 % (10 / 14 )。结论 除了检测SARS CoV病毒基因的F PCR方法以外 ,对疑似患者血清标本进行N蛋白的检测 ,具有早期预报意义。
Objective To compare the possible role of four kits for detecting SARS CoV genes, antigens and antibodies in early laboratory diagnosis of SARS patients. Methods SARS CoVIgG, SARS CoVIgM and SARS CoVN protein were detected by ELISA. The SARS CoV nucleic acid was detected by fluorescence quantitative polymerase chain reaction (F PCR). Results According to the results of 16 2 serum tests, the positive rate of N antigen was 90.2% (5 5/6 1) after onset for 1 to 5 days. The positive rates of IgG and IgM on day 15 to 18 after onset were 92 .8% (13/14). According to the results of 82 throat swabs, the positive rate of F PCR was 56.3% (14/2 4), 6-9 days Up to 71.4% (10/14). Conclusion In addition to the F PCR method for detecting the gene of SARS CoV virus, the detection of N protein in the serum of suspected patients has an early prediction significance.