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谷胱苷肽转移酶(glutathione-S-transferase,GST)是植物生长发育过程中一类多功能酶,在植物的初级代谢和次级代谢以及调控植物的逆境胁迫和细胞信号转导等过程中具有重要作用。根据GST蛋白序列构建的隐马尔可夫模型(hidden markov model,HMM),鉴定玉米全基因组GST基因,并对GST基因数量、类型、染色体定位和进化关系进行分析。结果表明,玉米全基因组中共含有37个GST基因,在10条染色体上呈非随机分布,具有U型、F型和Z型3个亚族。与水稻比较,玉米未发现T亚族。该研究结果对深入了解GST基因家族的分子进化以及GST基因的克隆和功能验证提供了重要参考依据。
Glutathione-S-transferase (GST) is a kind of multifunctional enzyme in the process of plant growth and development. It plays an important role in the primary metabolism and secondary metabolism of plants and in the regulation of stress tolerance and cell signal transduction of plants Has an important role. According to the hidden markov model (HMM) constructed based on the GST protein sequence, the GST gene of maize genome was identified and the relationship between GST gene number, type, chromosome location and evolution was analyzed. The results showed that 37 GST genes were found in the whole genome of maize and distributed non-randomly on 10 chromosomes. There were 3 subfamilies of U, F and Z genotypes. Compared with rice, corn did not find T subfamily. The results provide an important reference for further understanding of the molecular evolution of GST gene family and the cloning and functional verification of GST gene.