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目的:在建立小型猪下颌骨放射性骨坏死动物模型基础上,应用电镜技术研究下颌骨放射性骨坏死形成、发展的超微结构动态变化过程。方法:对6只小型猪右侧下颌骨进行25 Gy和28 Gy一次性照射,建立小型猪右侧下颌骨放射性骨坏死动物模型,分别于照射结束后3、4、5个月切取右侧下颌骨放射性骨坏死部位死骨,制备标本,进行电镜检查分析。结果:照射后的早期骨胶原纤维即受到破坏,骨细胞先出现细胞膜破坏,随后胞质出现空泡,细胞器裂解,最后出现核变化。大剂量照射后骨细胞的破坏出现早而重,并且加速了骨细胞的裂解、死亡。结论:骨细胞细胞膜及细胞质较细胞核对放射线更为敏感,照射后骨细胞死亡过程首先发生在细胞膜及细胞质内,与以往认为细胞核损伤在先的观点不同。
OBJECTIVE: To establish an animal model of mandibular osteoradodecrosis of the miniature pig based on electron microscopy and to study the dynamic process of ultrastructural changes in the formation and development of osteonecrosis of the mandible. Methods: The right mandibular bone of 6 miniature pigs was irradiated with 25 Gy and 28 Gy once. The animal model of mandibular radiating osteonecrosis of the right side of miniature pig was established. The right mandibular jaw was excised three, four and five months after irradiation Bone necrosis of the site of bone necrosis, preparation of specimens for electron microscopy analysis. Results: The early exposure to collagen fibers was destroyed, the first destruction of the cell membrane of the cell membrane, followed by vacuolar cytoplasm, organelle lysis, and finally nuclear changes. Osteoblasts are damaged sooner or later after high-dose irradiation, and the osteoblasts are accelerated and die. CONCLUSION: The cell membrane and cytoplasm of osteocytes are more sensitive to radiation than the nuclei. The process of osteocyte death occurs first in the cell membrane and cytoplasm after irradiation, which is different from the previous view that nuclear injury is the first.