论文部分内容阅读
目的:探讨热消融不全后残存肝癌细胞HepG2的生物学特性变化。方法:47℃热处理肝癌细胞株HepG2 5min,7.5min,10min,HepG2为正常对照组,倒置光学显微镜下观察热消融不全后残癌细胞的形态变化;(MTS法检测残癌细胞继续培养24h,48h,72h后的OD值,观察其增殖能力;Transwell法检测残留癌细胞迁移能力;残癌细胞进行单细胞培养10 d,观察克隆形成能力。结果:随着热处理时间的延长,发生凋亡的HepG2癌细胞增多;残留癌细胞在24h内的增殖能力增强,随后48h-72h时间段增殖速率降低;迁移能力随热处理时间延长增强,克隆形成能力随热处理时间延长减弱。结论:热消融不全后残存肝癌细胞HepG2生物学特性发生了变化,在一定时间内增殖速度增快,随后恢复至正常,随着热处理时间的延长其迁移能力增强,但克隆形成能力则下降。
Objective: To investigate the changes of biological characteristics of HepG2 cells after thermal ablation. Methods: HepG2 cells were treated with heat-treated HepG2 at 47 ℃ for 5min, 7.5min and 10min, respectively. HepG2 cells were normal control group. The morphological changes of residual cancer cells were observed with inverted light microscope. , And the OD value after 72h, the proliferation ability was observed, the migration ability of residual cancer cells was detected by Transwell method.The residual cancer cells were cultured in single cell for 10 days to observe the formation ability of colony.Results: With the prolongation of heat treatment, apoptotic HepG2 The proliferation of residual cancer cells increased within 24 h, then the proliferation rate decreased from 48 h to 72 h, the migration ability increased with the prolongation of heat treatment, and the clonogenic ability decreased with the prolongation of heat treatment.Conclusion: The biological characteristics of HepG2 cells changed with the increase of proliferation rate within a certain period of time, and then returned to normal. With the extension of heat treatment time, the migration ability was enhanced, but the clonogenic capacity decreased.