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本文用四参数模型表述十二指肠钩虫卵和美洲钩虫卵的发育与死亡率,它保持了Smith等人以前提出的有关模型的易控性,并对不同培养条件下钩虫卵的发育与死亡率进行了比较。病人粪便经筛滤冲淘后,在低温孵箱中漂浮50分钟,实验温度下离心标本,将浓集的虫卵沉淀倾入佩氏培养皿中。每数100个虫卵为一批,各批标本均在盛有2ml蒸馏水的方形塑料管中培养,上盖载玻片。孵箱以5种温度(15,20,25,30,35℃)保存培养物,定期
In this paper, the four-parameter model was used to describe the developmental and mortality rates of H. duodenalis eggs and C. occidentalis eggs. It maintained the ease of control of the model previously proposed by Smith et al. Development and mortality were compared. After excrement was screened by the patient, the sample was allowed to float in a low-temperature incubator for 50 minutes. Centrifugal samples were centrifuged at the experimental temperature and the concentrated pellet was poured into a Petri dish. Each batch of 100 eggs for a group of batches of specimens were filled with 2ml of distilled water in a square plastic tube culture, cover slides. Incubators were cultured at 5 temperatures (15, 20, 25, 30, 35 ° C) and periodically