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用限制性酶切片段长度多态性分析对沈阳地区７０例血清经巢式聚合酶链反应扩增ＨＢＶＤＮＡ阳性的乙型肝炎患者的Ｃ基因前Ｃ区１８９６位点进行分析。结果：（１）急性乙型肝炎前Ｃ区变异检出率为４％（１／２０），慢性迁延肝炎为２５％（５／２０），慢性活动性肝炎为３８％（５／１３），肝硬化为３３％（４／１２）；（２）ＡＬＴ升高组中，ＨＢｅＡｇ及抗ＨＢｅ均阴性患者和ＨＢｅＡｇ阴性，抗ＨＢｅ阳性患者ＨＢＶＤＮＡ前Ｃ区变异检出率分别为５７％和５０％，明显高于ＡＬＴ正常组（１７％和０％）。上述结果说明ＨＢＶ感染时间长短和肝功反复活动与ＨＢＶＤＮＡ前Ｃ区变异有关。
The C region pre-C 1896 locus in HBVDNA positive hepatitis B patients amplified by nested polymerase chain reaction in 70 cases in Shenyang were analyzed by restriction fragment length polymorphism. Results: (1) The detection rate of pre-C mutation in acute hepatitis B was 4% (1/20), chronic degenerative hepatitis was 25% (5/20), chronic active hepatitis was 38% (5/13) Cirrhosis was 33% (4/12). (2) The positive rate of pre-HBVDNA mutation in HBeAg-positive and HBeAg-negative patients and HBeAg-negative and HBe-positive patients in ALT elevated group were 57% and 50% , Significantly higher than the normal ALT group (17% and 0%). The above results show that the duration of HBV infection and repeated liver function associated with pre-HBVDNA mutation.