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目的 探讨人类精子染色体数目异常与自然流产的相关性。方法 采用多色荧光原位杂交(fluorescence in situ hybridization,FISH)方法 ,利用 CEP(chromesome enumeration DNA probes) X、Y、18及 L SI(locus specific identifier DNA probes) 13、2 1两种探针混合液与二硫苏糖醇处理过的自然流产者丈夫的精子核杂交 ,记数杂交信号 ,与对照组进行比较。结果 经卡方检验 ,自然流产组丈夫精子染色体 X、Y、18、13、2 1双体型及其它数目异常明显高于正常对照组 (P<0 .0 0 5 ) ,说明流产者丈夫精子染色体异常是流产的重要原因之一。结论 FISH技术是一种快速、准确、简单、实用性强的检测精子染色体的好方法。
Objective To investigate the correlation between the number of human sperm chromosome abnormalities and spontaneous abortion. Methods The fluorescence in situ hybridization (FISH) method was used in this study. CEP (chromesome enumeration DNA probes) X, Y, 18 and L SI (locus specific identifier DNA probes) Liquid and dithiothreitol-treated spontaneous abortion husband’s sperm nucleus hybridization, counting hybridization signals, compared with the control group. Results The chi square test showed that the abnormalities of X, Y, 18, 13 and 21 in the spontaneous abortion group were significantly higher than those in the normal control group (P <0.05), indicating that the abortion husbands husband sperm chromosome Abnormality is one of the important causes of abortion. Conclusion FISH is a fast, accurate, simple and practical method for detecting sperm chromosomes.