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Objective:To investigate the possible mechanism through which Artemisinin induced apoptosis in pancreatic cell line.Methods:Column chromatography,thin layer chromatography(TLC)and proton NMR spectroscopy were used to purify Artemisinin.The flowcytometry was employed to detect apoptosis and reactive oxygen species(ROS).Results:The results indicated that 50%inhibiting concentration(IC_(50)value)for pancreatic cell line(RIN)was 45μmol/L of Artemisinin.Artemisinin had no cytotoxic effect on the growth of peripheral blood lymphocytes.The mechanism of apoptosis was evaluated by measuring intracellular ROS.It was shown that Artemisinin-induced apoptosis occurred independently of the binding of CD95L to CD95 receptor in the RIN cells.Moreover,Artemisinin,in a dose-dependent manner,could significantly increase the level of ROS.Conclusion:Artemisinin can induce apoptosis in the RIN cells via the generation of ROS and triggering the intrinsic pathway of cell death.
Objective: To investigate the possible mechanism through which Artemisinin induced apoptosis in pancreatic cell line. Methods: Column chromatography, thin layer chromatography (TLC) and proton NMR spectroscopy were used to purify Artemisinin. Flowcytometry was employed to detect apoptosis and reactive oxygen species Results: The results indicated that 50% inhibiting concentration (IC 50 value) for pancreatic cell line (RIN) was 45 μmol / L of Artemisinin. Artemisinin had no cytotoxic effect on the growth of peripheral blood lymphocytes. The mechanism of apoptosis was evaluated by measuring intracellular ROS.It was shown that Artemisinin-induced apoptosis occurred independently of the binding of CD95L to CD95 receptor in the RIN cells. Moreover, Artemisinin, in a dose-dependent manner, could significantly increase the level of ROS. Conclusion: Artemisinin can induce apoptosis in the RIN cells via the generation of ROS and triggering the intrinsic pathway of cell death.