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为明确猕猴桃病毒A(Actinidia virus A,Ac VA)和猕猴桃病毒B(Actinidia virus B,Ac VB)在中国猕猴桃(Actinidia sp.)上的侵染状况和分子特性,采用RT-PCR技术对151份猕猴桃样品的Ac VA和Ac VB进行检测,检出率分别为15.2%和17.9%,所收集的6种猕猴桃样品中均检测到一种或两种病毒。发现Ac VA的ORF1、ORF4和ORF5扩增片段序列存在较大分子变异,不同分离物的269 bp片段(ORF1)核苷酸序列相似性为77.3%~99.3%。在基于各扩增片段核苷酸序列的系统进化树中,Ac VA分离物聚为2~3组。采用引物Ac VB5F/5R扩增获得20个Ac VB分离物的342 bp或338 bp的ORF5片段,核苷酸序列相似性为81%~100%,与新西兰Ac VB分离物TP7-93B相应片段的核苷酸序列相似性为83.9%~99.7%,在系统进化树中聚为3组。
In order to clarify the infection status and molecular characteristics of Actinidia virus A (Ac VA) and Actinidia virus B (Ac VB) in Actinidia sp., 151 Ac VA and Ac VB of kiwifruit samples were detected with the detection rates of 15.2% and 17.9%, respectively. One or two viruses were detected in the collected kiwifruit samples. It was found that there was a large molecular variation in the sequences of ORF1, ORF4 and ORF5 of Ac VA. The nucleotide sequence similarity of the 269 bp fragment (ORF1) of different isolates was 77.3% -99.3%. In the phylogenetic tree based on the nucleotide sequences of each amplified fragment, the Ac VA isolates clustered in 2 to 3 groups. A 342 bp or 338 bp ORF5 fragment of 20 Ac VB isolates was amplified by primer Ac VB5F / 5R. The nucleotide sequence similarity was 81% -100%, which was close to the corresponding fragment of TP7-93B of Ac VB isolate in New Zealand Nucleotide sequence similarity was 83.9% ~ 99.7%, and phylogenetic tree clustered into 3 groups.