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目的探讨两种天然药物三氧化二砷(ATO)和莪术(RC)对晶状体上皮细胞(LEC)增殖的抑制作用,为寻求防治后发性白内障的确切有效药物提供实验依据。设计实验性研究。研究对象体外培养的牛LEC。方法倒置显微镜下观察细胞形态学变化。MTT比色法检测不同终浓度(2．5、5、10μmol/L)ATO和(5、10、20mg/ml)RC作用于增殖状态下LEC 72小时后对细胞增殖的抑制程度。主要指标细胞形态、吸光度(A)值。结果倒置显微镜下可见增殖对照组细胞大小均匀,生长密集,ATO和RC组细胞生长及形态有明显改变,增殖缓慢,生长差,贴壁能力减弱。MTT比色法结果显示:不同浓度(2．5、5、 10μmol／L)的ATO和(5、10、20mg／ml)RC可明显抑制LEC增殖,并具有剂量依赖关系(P均<0．01)。结论两种天然药物 ATO、RC能够明显抑制LEC增殖,有望成为防治后发性白内障的有效药物。
Objective To investigate the inhibitory effects of two natural drugs, arsenic trioxide (ATO) and Rhizoma Curcuma (Roche), on the proliferation of lens epithelial cells (LECs) and to provide experimental evidence for the search for effective and effective drugs for the prevention and treatment of post-cataract. Design experimental research. Study object In vitro cultured cow LEC. Methods The morphological changes of cells were observed under inverted microscope. MTT assay was used to detect the inhibitory effect of RCT (2.5, 10, 10μmol / L) and RCT (5, 10, 20mg / ml) on the proliferation of LECs in proliferative state for 72 hours. The main indicators of cell morphology, absorbance (A) value. Results Under inverted microscope, the cell proliferation in control group was uniform and grew densely. The cell growth and morphology of ATO and RC groups changed obviously, with slow proliferation, poor growth and decreased adhesion. The results of MTT assay showed that ATO and (5,10 and 20 mg / ml) RCs at different concentrations (2.5, 10 and 10 mg / ml) significantly inhibited LEC proliferation in a dose-dependent manner (all P <0. 01). Conclusion Both natural drugs ATO and RC can significantly inhibit the proliferation of LEC, which is expected to become an effective drug for the prevention and treatment of post-cataract.