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Hyaluronan is a kind of polysaccharide with high molecule weight. Its structure is relatively complicated and tertiary structure exists among the HA molecules when reaching to a certain solution concentration. FT-IR spectrum has been acquired to show the groups and their ascription in HA. The results of FT-IR spectrum in this essay accords well with that issued by ASTM. As to the newly appearing absorption peaks, sensible explanation has been given. In addition, Ramon spectrum has been employed as a proof for the result of the FT-IR spectrum. Based on the preliminary acknowledge of the functional groups in HA, circular dichroism of the material has also been explored.