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目的研究人血管生成素1(Ang-1)、血管内皮生长因子165(VEGF165)以及两种因子相互作用对人胃癌细胞株MGC-803增殖与凋亡的影响。方法应用MTT法测定腺病毒绿色荧光蛋白(Ad-GFP)(B组)、Ad-Ang-1(C组)、Ad-VEGF165(D组)以及Ad-Ang-1+Ad-VEGF165/2(E组)对MGC-803细胞增殖的影响;另设对照组(A组)。采用流式细胞术分析血清饥饿时其对凋亡的影响;运用Western blot方法检测Bcl-2和Bax蛋白的表达。结果 24、48、72 h时,C、D、E组吸光度均明显高于B、A组(P<0.05);E组吸光度明显高于C、D组(P<0.05)。与A组或B组相比,C、D、E组均能够抑制细胞凋亡(P<0.01),其中E组抑制凋亡作用最强。C、D、E组Bcl-2蛋白表达均比B、A组明显增高(P<0.05),Bax蛋白的表达则明显降低(P<0.05)。结论 Ang-1、VEGF165和Ang-1+VEGF165/2能够明显促进MGC-803细胞体外增殖,抑制血清饥饿时的凋亡;Ang-1与VEGF165联合作用要显著强于单用Ang-1或VEGF165。
Objective To investigate the effects of Ang-1, VEGF165 and the interaction between two factors on the proliferation and apoptosis of human gastric cancer cell line MGC-803. Methods Ad-GFP (Ad-GFP), Ad-Ang-1 (group C), Ad-VEGF165 (group D) and Ad-Ang-1 + Ad-VEGF165 / 2 E group) on the proliferation of MGC-803 cells; another control group (A group). The effect of serum starvation on apoptosis was analyzed by flow cytometry. The expression of Bcl-2 and Bax protein was detected by Western blot. Results At 24, 48 and 72 h, the absorbance of group C, D and E were significantly higher than that of group B and A (P <0.05). The absorbance of group E was significantly higher than that of group C and D (P <0.05). Compared with group A or group B, group C, D and E inhibited apoptosis (P <0.01), and group E showed the strongest inhibitory effect on apoptosis. The protein expressions of Bcl-2 in groups C, D and E were significantly higher than those in groups B and A (P <0.05), while the expression of Bax was significantly decreased (P <0.05). Conclusions Ang-1, VEGF165 and Ang-1 + VEGF165 / 2 can significantly promote the proliferation of MGC-803 cells in vitro and inhibit apoptosis during serum starvation. The combined effect of Ang-1 and VEGF165 is significantly stronger than that of Ang-1 or VEGF165 alone .