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作者利用石蜡组织切片的HRP-SPA-GDN和HRP-SPA染色技术,对豚鼠胰腺生长抑素(somatostatin,SOM)和高血糖素(glucagon,Gluc)免疫反应细胞的分布及形态学作了初步观察。结果表明,SOM免疫反应细胞主要分布在胰岛的中央和周边部,而Gluc细胞则主要位于胰岛的周边部,胰岛内SOM免疫反应细胞形态各异,多数细胞伸出较长的细胞突起,而Gluc免疫反应细胞则形态均一,呈圆形或椭圆形。在胰外分泌部有散在分布的SOM阳性细胞,位于腺泡及导管上皮细胞间,或结缔组织中。在外分泌部木见到Gluc阳性细胞。
The authors first observed the distribution and morphology of somatostatin (SOM) and glucagon (Gluc) immunoreactive cells in guinea pigs using HRP-SPA-GDN and HRP-SPA staining techniques . The results showed that SOM immunoreactive cells mainly distributed in the central and peripheral part of islets, whereas Gluc cells mainly located in the peripheral part of islets. SOM immunoreactive cells in islets varied in shape, most of them extended longer protuberances, whereas Gluc Immunoreactive cells are uniform morphology, was round or oval. SOM-positive cells scattered in the extra-pancreatic secretions are located between the acinar and ductal epithelial cells or connective tissue. Gluc positive cells are seen in the exocrine section.