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A sensitive and specific ELISA for human thyrotropin(hTSH) is established with using two anti-hTSH monoclonal antibody. One of them is coated on the microtiter plate, the other is conjugate of biotin. The label is horseradish peroxidase(HRP) conjugate of streptavidin. TMB-H2O2 solution is used as the substrate of HRP. The sensitivity of the assay is 0.02 mIU/L. The intra-assay CVs and the intre-assay CVs of 3 samples are lower than 7.8% and 9.6%, respectively. The analytical recoveries are in the range from 93.3% to 107.8%. The assay is specific for hTSH and no cross reaction with other glyco-proteins, such as hLH , hHCG, hFSH. TSH concentrations range from 0.3 to 4,1 mlU/L in 142 normal subjects. As a reference IRMA(Multipact) method, the
One of them is coated on the microtiter plate, the other is conjugate of biotin. The label is horseradish peroxidase (HRP) conjugate of streptavidin The sensitivity of the assay was 0.02 mIU / L. The intra-assay CVs and the intre-assay CVs of 3 samples were lower than 7.8% and 9.6%, respectively. The TMB-H2O2 solution was used as the substrate of HRP. The assay is specific for hTSH and no cross reaction with other glyco-proteins, such as hLH, hHCG, hFSH. TSH concentrations range from 0.3 to 4, 1 mlU / L in 142 normal subjects. As a reference IRMA (Multipact) method, the