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从2001年WHO/IUATLD全球抗结核药物耐药监测之内蒙古耐药监测结核菌1 114株中,选取经比例法药敏结果得到的耐多药(MDR)结核菌188株,利用Geno Type MTBDRplus方法检测该批菌株的RIF、INH耐药情况及其耐药基因突变形式。最终得到MDR菌株103(54.79%)株,单耐RIF菌株52(27.66%)株,单耐INH菌株10(5.32%)株,全敏感菌株20株,TUB条带缺失1株,kat G质控带缺失2株。RIF耐药菌株检测的是rpo B基因区,该基因突变菌株有155株;rpo B S531L突变菌株为49.68%(77/155)。INH耐药菌株检测的是kat G基因区和inh A基因启动子区,共113株,其中kat G基因突变菌株占79.65%(90/113),主要是S3-15T1突变;inh A基因突变菌株占22.12%(25/113)。因此,Geno Type MTBDRplus可用于内蒙古地区MDR结核菌的快速检测。其中rpo BS531L突变形式在RIF耐药菌株中最常见;在INH耐药菌株中,kat G基因突变较inh A基因突变常见,S315T1突变是INH耐药菌中常见的突变形式。
188 strains of multidrug-resistant multidrug-resistant Mycobacterium tuberculosis (MDR) strains were screened from the drug susceptibility results by proportional method from 1,114 strains of drug-resistant Mycobacterium tuberculosis in Inner Mongolia in 2001, which were monitored by WHO / IUATLD global anti-TB drug resistance test. Genotype MTBDRplus The strains were tested for RIF, INH resistance and mutation patterns of drug-resistant genes. The results showed that MDR strain 103 (54.79%), single RIF resistant strain 52 (27.66%), single resistant INH strain 10 (5.32%), total sensitive strain 20, Two strains with deletion. RIF resistant strains detected rpo B gene region, the gene mutation strains 155; rpo B S531L mutant strains 49.68% (77/155). INH resistant strains detected 113 kat G and inh A promoter regions, of which kat G gene mutation accounted for 79.65% (90/113), mainly S3-15T1 mutation; inh A gene mutation strain Accounting for 22.12% (25/113). Therefore, Geno Type MTBDRplus can be used for the rapid detection of Mycobacterium tuberculosis in Inner Mongolia. Among them, the mutation of rpo BS531L was the most common in RIF-resistant strains. In INH-resistant strains, kat G gene mutation was more common than inh A gene mutation, and S315T1 mutation was a common mutation in INH-resistant bacteria.