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The strain CCH, isolated from the surface of the plant flower in China, is a wall- less,helical prokaryote which passes through a microfilter 0. 22 μm in diameter. Cholesterol isrequired for its growth. The strain CCH could metabolize glucose, fructose, maltose, treha-lose and arginine, but not urea. Its electrophoresis pattern of cell proteins is distinct fromthat of other spiroplasmas. The guanine-plus-cyrosine content of its DNA determined bymelting temperature is 29. 15 mol%. No relation with any known spiroplasmas was found inenzyme-linked immunosorbent assay and deformation tests. According to these results, thestrain CCH is referred to a new species in the genus of Spiroplasma or the representative ofa new spiroplasma group.
The strain CCH, isolated from the surface of the plant flower in China, is a wall- less, helical prokaryote which passes through a microfilter 0.22 μm in diameter. Cholesterol isrequired for its growth. The strain CCH could metabolize glucose, fructose, maltose, treha-lose and arginine, but not urea. Its electrophoresis pattern of cell proteins is distinct from that of other spiroplasmas. The guanine-plus-cyrosine content of its DNA determined by melting temperature is 29. 15 mol%. No relation with any known spiroplasmas was found inenzyme-linked immunosorbent assay and deformation tests. According to these results, the strain CCH is referred to a new species in the genus of Spiroplasma or the representative of a new spiroplasma group.