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目的:观察实验性阻塞性肺气肿大鼠的凝血纤溶系统失衡状态,并对其机制进行探讨。方法:Wistar大鼠24只,随机分为2组,正常对照组和模型组,每组12只。采用熏香烟加尾静脉注射内毒素的方法制备实验性阻塞性肺气肿大鼠模型。实验结束后,观察各组大鼠的病理及肺功能情况,用酶联免疫吸附法(ELISA)测定血浆组织型纤溶酶原激活剂(tPA)、纤溶酶原激活物抑制剂-1(PAI-1)、组织因子途径抑制物抗原(TFPI:Ag)的含量,发色底物法测定血浆抗凝血酶-Ⅲ(AT-Ⅲ)活性。结果:模型组大鼠肺功能指标第0·2s用力呼出容积占用力肺活量百分比(FEV0·2/FVC)(68·25±8·90)%,与正常对照组(88·52±2·09)%比较,差异有显著性(P<0·01);模型组大鼠AT-Ⅲ和TFPI分别为(56·04±14·81)%、(1·39±0·26)ng/mL,与正常对照组(85·46±18·03)%、(1·05±0·17)ng/mL比较,差异有显著性(P均<0·01);模型组大鼠tPA和PAI-1分别为(2·1±0·73)ng/mL、(0·54±0·07)ng/mL,与正常对照组(1·45±0·43)ng/mL、(0·80±0·24)ng/mL比较,差异有显著性(P均<0·05)。结论:模型组大鼠体内的凝血纤溶系统存在失衡状态,AT-Ⅲ、TFPI、tPA和PAI-1可能参与这一过程。
OBJECTIVE: To observe the imbalance of coagulation and fibrinolytic system in experimental obstructive emphysema in rats and to explore its mechanism. Methods: Twenty-four Wistar rats were randomly divided into 2 groups, normal control group and model group, with 12 rats in each group. A rat model of experimental obstructive pulmonary emphysema was established by injecting endotoxin with cigarette and tail vein. At the end of the experiment, the pathological and pulmonary function of the rats in each group were observed. The levels of plasma tissue plasminogen activator (tPA), plasminogen activator inhibitor-1 PAI-1, TFPI: Ag, and the chromogenic substrate method for the determination of plasma antithrombin-III (AT-III) activity. Results: The FEV0.2 / FVC (68.25 ± 8.90)% of the FEV0.2s pulmonary function index of the model group was significantly higher than that of the normal control group (88.52 ± 2.09) )%, The difference was significant (P <0.01). The AT-III and TFPI of model group were (56.04 ± 14.81)% and (1.39 ± 0.26) ng / mL respectively , Compared with the normal control group (85.46 ± 18.03)%, (1.05 ± 0.17) ng / mL, the difference was statistically significant (all P <0.01). The levels of tPA and PAI -1 were (2.1 ± 0.73) ng / mL, (0.54 ± 0.07) ng / mL, respectively, which were significantly higher than those in the normal control group (1.45 ± 0.43 ng / mL, 80 ± 0.24 ng / mL, the difference was significant (P <0.05). CONCLUSION: The coagulation-fibrinolytic system in rat model is imbalanced. AT-Ⅲ, TFPI, tPA and PAI-1 may be involved in this process.