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目的探讨人参二醇组皂苷(panoxadiol saponin PDS)与顺铂(cisplatin,DDP)联合应用对DU145前列腺癌细胞增殖及凋亡的影响。方法采用MTT比色法检测PDS与DDP联合应用对DU145细胞增殖活力的影响;吖啶橙染色观察诱导细胞凋亡情况;流式细胞仪分析细胞周期及凋亡,并计算细胞平均凋亡率;免疫化学和Western blot技术观察细胞内激活的caspase3的表达。结果100mg/L PDS与0.2μmol/L DDP联合给药:(1)48h后可使单独应用0.2μmol/L DDP组其肿瘤细胞生长抑制率由16.35%提高到47.13%;(2)吖啶橙荧光染色可见细胞呈现明显凋亡形态,其凋亡率与2μmol/L DDP组相当;(3)流式细胞术结果显示,可使单独应用0.2μmol/L DDP组其诱导DU145细胞凋亡率从5.53%提高到19.39%,相当于其10倍剂量即2.0μmol/L DDP的诱导凋亡效应(21.05%),明显高于PDS单独应用的凋亡率;(4)免疫化学和Western blot结果显示,可使单独应用0.2μmol/L DDP组细胞内激活的caspase3阳性细胞率与2μmol/L DDP组相当。结论PDS能增强DDP对DU145前列腺癌细胞的致凋亡效应。
Objective To investigate the effects of panaxadiol saponin PDS combined with cisplatin (DDP) on the proliferation and apoptosis of DU145 prostate cancer cells. Methods MTT assay was used to detect the effect of PDS combined with DDP on the proliferation activity of DU145 cells. The apoptosis of DU145 cells was observed by acridine orange staining. The cell cycle and apoptosis were analyzed by flow cytometry and the average apoptosis rate was calculated. Immunocytochemistry and Western blot were used to observe the expression of intracellular activated caspase3. Results Combined administration of 100 mg / L PDS and 0.2 μmol / L DDP: (1) After 48 h, the growth inhibition rate of tumor cells in the group of 0.2 μmol / L DDP alone was increased from 16.35% to 47.13%; (2) Fluorescence staining showed that the cells showed obvious apoptotic morphology and the apoptosis rate was similar to that of 2 μmol / L DDP group. (3) Flow cytometry results showed that the apoptosis rate of DU145 cells induced by 0.2 μmol / L DDP alone 5.53% to 19.39%, which was equivalent to the 10-fold dose of 2.0μmol / L DDP induced apoptosis (21.05%), which was significantly higher than that of PDS alone. (4) Immunochemistry and Western blot showed , The intracellular activation of caspase 3-positive cells in 0.2 μmol / L DDP alone group was comparable to that of 2 μmol / L DDP group. Conclusion PDS can enhance the apoptotic effect of DDP on DU145 prostate cancer cells.