论文部分内容阅读
为探讨运动负荷引起机体适宜的酶活性反应指标 ,以防止因过度负荷产生自由基的增加 ,降低机体抗氧化能力而造成对机体的损害 ,采用硫代巴比妥酸法和化学发光分析法对不同负荷跑台训练后大鼠脑组织内MDA含量和总SOD、胞浆SOD(CuZn -SOD)及线粒体SOD(Mn -SOD)酶活性进行测定。结果表明 ,一般负荷训练 3周组大鼠脑组织内MDA含量明显高于对照组 (P <0 0 5 ) ,5周、7周组变化不明显。极限负荷训练组显著高于对照组 ,极限 7周组极显著性高于 3周、5周组和一般负荷训练 7周组 ;一般负荷训练组的脑SOD酶活性明显高于对照组。极限负荷训练组与对照组之间变化不明显 ,但明显低于一般负荷训练组 ,可能与机体疲劳有关 ;各组大鼠脑组织胞浆内的CuZn -S0D酶活性与总SOD酶活性之间存在着密切的正相关。而线粒体Mn -SOD与总SOD酶活性之间无明显的相关性。研究结果提示 :长期超负荷训练可引起机体自由基产生增加 ,可能会降低机体的抗氧化能力 ,因而导致组织细胞膜结构损伤或机体疲劳。适量的耐力负荷训练可改善机体抗自由基损伤能力。
In order to explore the appropriate exercise-induced enzyme activity of body response indicators to prevent over-load to produce free radicals, reduce the body’s antioxidant capacity and cause damage to the body, the use of thiobarbituric acid and chemiluminescence analysis The contents of MDA and the contents of total SOD, the activities of SOD (CuZn-SOD) and mitochondrial SOD (Mn-SOD) in brain tissue of rats after treadmill training at different loads were measured. The results showed that the content of MDA in the brain tissue of the rats in the 3-week training group was significantly higher than that in the control group (P <0.05). The changes of 5-week and 7-week groups were not obvious. Compared with the control group, the exercise load in the extreme load training group was significantly higher than that in the control group, the extreme 7-week training group was significantly higher than the 3-week, 5-week training group and general-load training 7-week training group. There was no significant difference between the limit load training group and the control group, but it was significantly lower than that of the general load training group, which may be related to the body fatigue; between the CuZn-SOD and total SOD enzyme activities in the cytoplasm There is a close positive correlation. There was no significant correlation between mitochondrial Mn-SOD and total SOD activity. The results suggest that long-term overloaded training can cause the body to produce free radicals, which may reduce the body’s antioxidant capacity, resulting in structural cell membrane damage or body fatigue. Moderate endurance load training can improve the body’s ability to resist free radical damage.