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目的探讨整合蛋白α５亚基与原发性肝癌的关系。方法应用免疫组化技术（ＡＢＣ法）和Ｎｏｒｔｈｅｒｎｂｌｏｔ杂交检测整合蛋白α５亚基在原发性肝癌中的表达。结果发现在７９例癌与癌周组织α５阳性率分别为３２．９％和８１．０％，两者间差异有显著性（Ｐ＜０．０１）。直径≤５ｃｍ的肝癌α５阳性率高于直径＞１０ｃｍ的肝癌（５５．６％比１０．０％，Ｐ＜０．０１），分化较好的肝癌α５阳性率高于分化不良者（４０．６％比１６．０％，Ｐ＜０．０５），已发生明确肝内转移（包括肝内播散和门静脉癌栓形成）的肝癌α５阳性率低于未发生肝内转移者（２０．６％比４２．２％，Ｐ＜０．０５）。α５亚基表达与患者年龄、血清甲胎蛋白水平、乙型肝炎病毒感染、肝硬化有无等因素均无明显相关（Ｐ＞０．０５）。Ｎｏｒｔｈｅｒｎｂｌｏｔ杂交结果也同时显示，非侵袭性肝癌α５表达高于侵袭性肝癌。结论整合蛋白α５低表达与肝癌增大、分化程度低、侵袭转移发生等恶性表型相关，可能对这些恶性表型起负性调节作用。
Objective To investigate the relationship between integrin α5 subunit and primary liver cancer. Methods Immunohistochemistry (ABC method) and Northern blot were used to detect the expression of integrin α5 subunit in primary liver cancer. The results showed that the positive rates of α5 in cancer tissues and cancer tissues were 32.9% and 81.0%, respectively, and there was a significant difference between them (P<0.01). The positive rate of α5 in hepatocellular carcinoma with diameter ≤5cm was higher than that in hepatocellular carcinoma with diameter >10cm (55.6% vs 10.0%, P<0.01). The positive rate of α5 in hepatocellular carcinoma with better differentiation was higher than that in poor differentiation (40.6). % (16.0%, P<0.05), and the positive rate of α5 in hepatocellular carcinoma with definite intrahepatic metastasis (including intrahepatic dissemination and portal vein tumor thrombus formation) was lower than those without intrahepatic metastasis (20.6%). 42.2%, P<0.05). The expression of α5 subunit was not significantly associated with the age of patients, serum alpha-fetoprotein levels, hepatitis B virus infection, and liver cirrhosis (P>0.05). Northern blot hybridization results also showed that non-invasive liver cancer α5 expression was higher than invasive liver cancer. Conclusion The low expression of integrin α5 is associated with malignant phenotypes such as hepatocellular carcinoma enlargement, low degree of differentiation, and invasion and metastasis, which may negatively regulate these malignant phenotypes.